Ultra Fast Time Resolved Spectrofluorometer

提出了一种快速、高灵敏度、时间分辨的荧光分光计，拟发展为商业项目。与时间相关单光子计数和相位调制等经典荧光测量方法不同，该仪器采用了两种最新发展的技术：a)单脉冲激光激发和纳秒电子成像波形分析；b)声光可调滤波器作为电控单色仪。该仪器能够在1秒内同时获得一套完整的衰减和光谱曲线，足以构建三维流形。仪器的灵敏度可高达飞摩尔（10-15摩尔）和原子摩尔（10-18摩尔）水平。这些都远远超出了现有工具的能力。该仪器结构紧凑，重量轻，成本低，适合并将促进荧光检查方法的广泛应用。第一阶段将演示可行性，第二阶段将开发原型。长期目标是使用该方法开发用于基础研究以及临床和工业应用的许多专门仪器。

A fast and high sensitivity time-resolved spectrofluorometer is proposed to develop into a commercial project. In contrast with the classical fluorometric method, such as time-correlated single photon counting and phase modulation, the novel instrument is based on the introduction of two recently developed techniques: a) single pulse laser excitation and nanosecond waveform analyzing by electronic imaging, and b) acoustooptical tunable filter as an electronically controlled monochromator. The instruments have the capability of simultaneously acquiring a complete set of decay and spectral curve sufficient for the construction of a 3-D manifold within 1 second. The sensitivity of the instrument can be as high as at femtomole (10-15 mole) and attomole (10-18 mole) level. These are far beyond the capabilities of current instruments. The instrument is compact, lightweight, low-cost, suitable for and will facilitate wide applications of the fluoroscopic method. Phase I will demonstrate the feasibility and Phase II will develop a prototype. The long-term goal is to use the method to develop numerous specialized instruments for basic research as well as for clinical and industrial applications.