Automated Analysis of Virus Populations

9309449 Yin本研究项目旨在开发一种同时从异质种群中分离和表征病毒克隆的方法。该方法的原理源于最近的理论结果，该结果表明，通过测量病毒株的繁殖速率对宿主密度的依赖，可以在培养皿中“指纹化”病毒株。指纹的形状和振幅中包含了反映特定菌株与其宿主相互作用的丰富详细信息，即其吸附率、复制率和每个受感染宿主的产量。设计并构建了指纹图像采集与分析系统。＊＊＊

9309449 Yin This research project is directed towards the development of a method to simultaneously isolate and characterize virus clones from a heterogeneous population. The principle of the method stems from recent theoretical results suggesting virus strains can be "fingerprinted" in a petri dish by measuring the dependence of their propagation rates on the density of their host. Embedded in the shape and amplitude of a fingerprint are a wealth of detailed information reflecting a particular strain's interaction with its host, i.e., its adsorption rate, replication rate and yield per infected host. An image acquisition and analysis system is to be designed and built to measure fingerprints. ***