Determination of the Molecular Basis of Biological Recognition at Functionalized Interfaces

在这个项目中，支持表面对受体结合率和与固定配体的结合亲和力的精确分子影响将被阐明。受体结合的动力学模型将被开发，包括相互作用的分子细节。受体与功能化表面的动力学和平衡结合将通过：(1)倏逝光纤荧光光谱进行研究；(2)定量荧光显微镜；(3)放射性标签。受体的吸附和解吸速率以及结合亲和力将由倏逝光纤光谱测定。特异性受体结合的均匀性将通过荧光显微镜进行评估。与功能化平面和囊泡的结合亲和力将用放射性标记的蛋白质来确定。

With this project, the precise molecular influences of supporting surfaces on receptor association rates and binding affinities to immobilized ligands are to be elucidated. Kinetic models of receptor binding are to be developed which incorporate the molecular details of interactions. The kinetics and equilibrium binding of receptors to functionalized surfaces will be studied by: (1) evanescent fiber optic fluorescence spectroscopy; (2) quantitative fluorescence microscopy; and (3) radiolabelling. Receptor adsoption and desorption rates and binding affinities will be determined by evanescent fiber optic spectroscopy. The uniformity of specific receptor binding will be assessed by fluorescence microscopy. Binding affinities to both functionalized planar surfaces and vesicles will be determined with radiolabelled proteins.