U.S. GLOBEC: Analysis of Short-Term Growth in Copepods and Larval Fish Using Molecular Markers of Cell Proliferation

9313676斯蒂格曼将微观和广泛的物理因素与甲鱼类桡足类、幼体鳕鱼和黑线鳕的生长联系起来，这对美国-GLOBEC乔治银行计划至关重要。以前测量生长的方法包括形态计量学、生活期持续时间、产蛋量和间接生化方法，如RNA/DNA比率。Stegeman博士将利用免疫组织化学、组织学和荧光测量方法来检测细胞增殖，继续将这项技术应用于海鱼和无脊椎动物。该方法使用针对合成核DNA的细胞的分子标记的单抗，以允许对此类细胞进行量化。早期的目标将是确定哪些细胞和器官的增殖率最接近地反映总体生长速度，在幼鱼中通过形态计量学测量，在桡足类中通过阶段长度测量。可以测量这种差异的时间尺度将在一系列实验室实验中进行研究。随后，他们将参加正在规划的美国-GLOBEC进程巡航，以开发和应用该方法在海上。参与这项研究将把由于短期和长期气候变化而波动的垂直混合和层化的物理测量转化为对这些参数的生物影响的即时分析。***

9313676 Stegeman Connecting micro- and broad-scale physical factors to growth in calanoid copepods and larval cod and haddock is essential to the US-GLOBEC Georges Bank Program. Prior methods to measure growth have involved morphometrics, life stage durations, egg production, and indirect biochemical methods, such as the RNA/DNA ratio. Dr. Stegeman will utilize immunohistochemical, histological and fluorometric methods to detect cell proliferation, continuing the application of this technique in marine fish and invertebrates. The method uses monoclonal antibodies to molecular markers of cells synthesizing nuclear DNA to allow quanitification of such cells. An early aim will be to establish which cell and organ proliferation rates most closely reflect overall growth rate, as measured morphometrically in larval fish, and by stage length in copepods. The time scale over which such differences can be measured will be studied in a series of laboratory experiments. Subsequently, they will participate in the US-GLOBEC Process cruises that are being planned, to develop and apply the method at sea. Participation in this study will translate physical measurements of vertical mixing and stratification, that fluctuate as a result of short and long term climatic changes, into an instant assay for the biological effects of such parameters. ***