A Molecular Genetic Analysis of Transcription Termination inYeast

转录终止被定义为新生转录物的释放和RNA聚合酶从模板上的解离，可以说是真核生物转录过程中最不为人所知的一个方面。本研究的目的是帮助确定RNA聚合酶II终止转录的机制。有两个具体目标。第一篇描述了在体内指导3'端形成所需的序列的实验。提出次级结构可能对这次事件很重要的模型将得到检验。第二个目的是利用体外生化测定来补充和扩展分子方法。分子生物学中许多研究的重点是对基因表达的研究。这涉及到基因如何在某些细胞类型中开启，而在其他细胞类型中却没有。这项工作是关于基因表达的一个重要步骤——转录是如何被控制的。具体来说，我们会问负责转录反应的酶是如何在基因中适当的位置停止的。这个反应被称为转录终止。我们对转录终止知之甚少，但它是细胞基本机制的一个极其重要的组成部分。＊＊＊

9316701 Hyman Transcription termination, defined as the release of the nascent transcript and dissociation of RNA polymerase from the template, is arguably the least well understood aspect of eukaryotic transcription. The goal of the proposed research is to contribute to defining the mechanism(s) that are responsible for termination of transcription by RNA polymerase II. There are two specific aims. The first describes experiments that address which sequences are required to direct 3' end formation in vivo. Models proposing that a secondary structure may be important for this event will be tested. The second aim is to utilize an in vitro biochemical assay to complement, and extend the molecular approach. %%% The focus of much research in molecular biology is in the study of gene expression. This is concerned with how genes are turned on in some cell types but not others. This work is concerned with how transcription, an important step in gene expression, is controlled. Specifically we will ask how the enzyme which is responsible for the transcription reaction stops at the appropriate place in the gene. This reaction is referred to as transcription termination. Little is known about transcription termination yet it is an extremely important part of fundamental cell mechanisms. ***