MALD-TOF Mass Spectrometer for a Core Laboratory

9318193 Hogue-Angeletti我们申请资金，为一个具有合作和生产力记录的核心实验室购买一台基质辅助激光解吸、飞行时间质谱（MALD-TOF MS）仪器。该仪器将使阿尔伯特·爱因斯坦医学院的一组研究人员能够以高灵敏度确定蛋白质和糖蛋白的质量。除了对蛋白质样品进行常规分析以评估分子量、纯度和验证结构外，MALD-TOF质谱仪还将用于：1）鉴定参与分泌颗粒形成和蛋白质分选机制的蛋白质; 2）研究加工酶的加工; 3）鉴定室管膜和脉络丛细胞分泌的蛋白质和肽; 4）表征微管上的紫杉醇结合位点; 5）研究ATP依赖性蛋白酶的机制; 6）分析响应于集落刺激因子-1的酪氨酸快速磷酸化的蛋白质。

9318193 Hogue-Angeletti We request funds toward the purchase of an instrument for matrix-assisted laser desorption, time-of-flight mass spectrometry (MALD-TOF MS) for a core laboratory with an established record of cooperation and productivity. This instrument will enable a group of investigators at Albert Einstein College of Medicine to determine protein and glycoprotein masses at high sensitivity. In addition to routine analysis of protein samples to assess molecular weight, purity, and to verify structures, the MALD-TOF mass spectrometer will be used to: 1) identify proteins involved in the formation of secretory granules and in protein sorting mechanisms; 2) study the processing of processing enzymes; 3) identify proteins and peptides secreted by ependyma and choroid plexus cells; 4) characterize the taxol binding site on microtubules; S) study the mechanism of ATP- dependent proteases; 6) analysis of proteins rapidly phosphorylated in tyrosine in response to colony stimulating factor-l.