Kinetic Studies and Modelling of Interactions of Nutrient, Hormone, Growth Rate and Form Change in Carrot Embryo Development

9321426胡这个项目正在研究胡萝卜细胞悬浮液中的胚胎发育。需要检验的假设是，从球形胚状体到极地结构的转变是由营养和/或激素梯度引发的，这些梯度是由于对质量转移的抵抗而引起的。这项研究使用的技术包括成像算法、计算模型和利用之前的NSF拨款开发的实验协议。将检测钾、硝酸盐、氧气、葡萄糖和生长素对胚胎发育的影响。这项研究可能会对大规模繁殖的体细胞胚胎的受控生长产生影响。***

9321426 Hu This project is on research to examine embryogenic development in carrot cell suspensions. The hypothesis to be tested is that the transition from a spherical embryoid to a polar structure is triggered by nutrient and/or hormone gradients caused by resistance to mass transfer. The techniques used for this investigation include algorithms for imaging, computational models, and experimental protocols developed with a previous NSF grant. The effects of potassium, nitrate, oxygen, glucose, and auxin on embryogenesis will be examined. This research could have an impact on the controlled growth of somatic embryos for large scale propagation. ***