Macromolecular Crystallography Course, Cold Springs Harbor Laboratory, NY (October, 1994, 1995, & 1996)

9404782 Pflugrath大分子晶体学实训课程每年将培养16名学生，通过讲座和实际实验，对大分子晶体学的理论和实践方面进行培训。通过这种训练，学生们就可以在他们自己的结构生物学研究中使用晶体学技术。这种完整的晶体学沉浸式训练在其他地方是无法获得的。四名主要讲师和大约12名额外的演讲者进行了大量的讲座，指导学生进行实验室实验和计算，最终确定了一种实际蛋白质的完整结构-蛋清溶菌酶的129残基。具体实验包括蛋白质结晶、重原子衍生化、晶体安装和冷冻、空间群测定、衍射数据收集和分析、多重同构置换和分子置换相测定、帕特森和傅立叶变换计算、溶剂倾斜、异常散射、相组合、电子密度图解释、约束最小二乘细化、不同的傅里叶分析和结果的表示。讲师还介绍了他们自己实验室最近确定的结构，重点是使用的方法。此外，鼓励学生携带自己的蛋白质和数据来尝试课程中可用的技术和软件。课程内容每年进行评估和更新，以便与晶体学的最新发展保持同步。＊＊＊

9404782 Pflugrath The training course in Macromolecular Crystallography will train 16 students yearly in both the theoretical and the practical aspects of macromolecular crystallography through lectures and actual experiments. With this training, students are then prepared to use crystallographic techniques in their own structural biological research. Such complete immersive training in crystallography is unavailable elsewhere. Four principal instructors along with about 12 additional speakers give numerous lectures that guide students through laboratory experiments and computations which ultimately lead to the complete structure determination of an actual protein - the 129 residue hen egg white lysozyme. Specific experiments are performed in protein crystallization, heavy atom derivatization, crystal mounting & freezing, space group determination, diffraction data collection & analysis, phase determination by both multiple isomorphous replacement & molecular replacement, Patterson & Fourier transform calculation, solvent flattering, anomalous scattering, phase combination, electron density map interpretation, restrained least squares refinement, different Fourier analysis and presentation of results. The instructors also present structures recently determined in their own laboratories with an emphasis on the methods used. In addition, students are encouraged to bring their own protein and data to try out the techniques and software available during the course. The course curriculum is evaluated and updated annually in order to remain abreast of the latest development in crystallography. ***