Microanalytical Methods in Neurochemistry

这项研究项目得到了分析和表面化学计划的支持，涉及将窄口径毛细管中的毛细管电泳法与灵敏和选择性的检测方法相结合，以解决细胞神经科学中的几个独特实验。该项目的三个基本目标是开发超低容量分离方法来动态和选择性地跟踪单个神经细胞、生长锥和突触的化学释放；发展毛细管电泳质谱联用来定量培养中整个细胞膜、质膜区域切片和轴突生长锥中的磷脂；以及开发识别和定量单个发育中的生长锥中的化学信使的方法。这些技术将应用于神经生物学的两个重要领域，即通过研究生长锥体的神经发育和通过膜上的事件进行细胞间通信。该项目将在尤因教授和他的学生在国家科学基金会CHE-9106832资助期间取得的成果的基础上进行的分析方法学开发，对于在单细胞水平上实现维度和分子选择性神经化学测量非常重要。这些一般方法在生物技术中也很重要，并有望为环境研究的基本工具的发展做出贡献

This research project, supported in the Analytical and Surface Chemistry Program, concerns the combination of capillary electrophoresis in narrow-bore capillaries with sensitive and selective detection methods to address several unique experiments in cellular neuroscience. The three fundamental goals of this project are to develop ultra-low volume separation methods to dynamically and selectively follow chemical release from single nerve cells, growth cones, and synapses; to develop capillary electrophoresis-mass spectrometry to quantify phospholipids in membranes of whole cells, regional sections of the plasma membrane, and axonal growth cones in culture; and to develop methods to identify and quantify chemical messengers in single developing growth cones. These techniques will be brought to bear on two important areas of neurobiology, namely neural development through the study of growth cones and cell-to-cell communication through events at the membrane. The analytical methodology development to be pursued in this project, which builds upon results obtained by Professor Ewing and his students during the tenure of NSF grant CHE-9106832, is important to the achievement of dimensionally and molecularly selective neurochemical measurements at the single cell level. These general methodologies are also important in biotechnology and can be expected to contribute to the development of essential instrumentation for environmental studies