Recombinant Production of Hyprthermophilic Enzymes: Effects of Temperature and Pressures

9410687 Robb这个项目是研究利用重组脱氧核糖核酸技术，通过在嗜中性菌中表达这些酶来从极端菌中产生酶。目标是提供大量这些酶用于生物物理和生化表征，并最终用于酶的应用，而不需要直接培养极端细菌。本研究的具体目标包括：(1)克隆和鉴定嗜热嗜热菌和相关嗜热菌的基因序列；(2)在高压下在异源系统中表达基因，并在高压和温度下对重组酶进行特性鉴定，以建立最佳的比活性和组装；(3)开发生物处理方法以获得足够数量的酶，用于结构测定和生物技术应用。***

9410687 Robb This project is on research to study the production of enzymes from extremophiles by expression of these enzymes in mesophiles, using recombinant DNA technology. The objective is to make available large quantities of these enzymes for biophysical and biochemical characterization and ultimately for enzymatic applications, without the need for culturing extremophiles directly. The specific objectives of this research include: (1) to clone and identify gene sequences from the hyperthermophile Pyrococcus furiosus and related thermophiles; (2) to express genes in heterologous systems at high pressure, and characterize recombinant enzymes under elevated pressure and temperature to establish optimal specific activity and assembly; and (3) to develop bioprocessing methods for obtaining enzymes in sufficient quantity for structural determination and biotechnological applications. ***