Acquisition of: A Cytophotometry Facility for Direct Measurement of Nuclear DNA Content

我们打算在圣奥拉夫生物系建立一个细胞光度仪设施，用于研究和培训目的。这样的设备需要一台配备光源和滤光片的显微镜，以允许在两个波长测量吸光度，一台显微镜图像处理器和光度计。细胞热计将使我们能够对核DNA含量进行精确的定量测量。这种能力将极大地扩展我们的研究工作，以分析核和染色体在正常和异常结合的原生动物嗜热四hvmena。具体来说，我们将能够更好地表征影响减数分裂和有丝分裂的共轭突变体的核行为，以及新形成的多倍体大核的分化。

ABSTRACT It is our intention to establish a cytophotometry facility in the St. Olaf Biology Department for both research and training purposes. Such a facility requires a microscope equipped with a light source and filters that allow for absorbency measurements at two wavelengths, a microscope image processor, and a photometer. A cytophotometer will allow us perform accurate, quantitative measurements of nuclear DNA content. This capability will greatly expand upon our research efforts to analyze nuclear and chromosomal behavior during both normal and aberrant conjugation in the ciliated protozoan Tetrahvmena thermophila. Specifically, we will be better able to characterize the nuclear behavior of conjugation mutants that affect both meiotic and mitotic divisions, as well as the differentiation of newly formed polyploid macronuclei.