Acquisition of DNA Sequencer

在本提案中，我们申请资助perkins - elmer / Applied Biosystems自动测序仪型号373A-01。这台机器将主要由四个实验室组成的小组使用，也将向植物生物系和环境科学、政策和管理系的其他用户开放。以前使用借来的机器的经验表明，我们会有效地利用它。空间、支持人员和集体资源可用于机器的永久住房和维护。目前伯克利校区没有核心测序设备。这台机器将使校园里的永久机器数量增加一倍，并且是两个系的成员唯一可以使用的机器。使用借来的自动测序仪加快了我们开展目前美国国家科学基金会资助工作的速度，也促进了新方法的创新，或扩大了微生物生态学、群体遗传学、植物发育和植物抗病领域的先前期望。很明显，自动测序将很快成为实验室的首选方法，比如我们的实验室，序列获取是一个限制速度的步骤，因此，它也是研究生和博士后同事应该学习的方法。

In this proposal we request funding for a Perkin-Elmer / Applied Biosystems Automated Sequencer model 373A-01 . This machine will be used primarily by a group of four labs and will be also open to other users in the Department of Plant Biology and the Department of Environmental Science, Policy, and Management. Prior experience with a loaned machine demonstrates that we will make efficient use of it. Space, support staff, and collective resources are available for permanent housing and maintenance of the machine. There is currently no core sequencing facility on the Berkeley campus. This machine would double the number of permanent machines on campus and would be the only one available to members of either department . Access to borrowed automated sequencer has accelerated the rate at which we conduct our current NSF sponsored work and has also facilitated innovations of new approaches or expansions of prior expectations in the fields of microbial ecology, population genetics, plant development, and plant disease resistance. It is clear that automated sequencing will soon be the method of choice for labs such as ours in which sequence acquisition is a rate limiting step, and as such it is also the method that graduate students and postdoctoral associates should learn.