U.S.-Czech Research on Flow Karotyping and Sorting of Translocations in Maize

小行星9423154 这个美国-亚利桑那大学的大卫·加尔布雷斯博士和捷克实验植物研究所的雅罗斯拉夫·多莱泽尔博士之间的捷克研究项目将研究分生组织根尖细胞的细胞周期同步化程序，从停滞在中期的同步化细胞中分离浓缩染色体，以及这些染色体的流式分选。 这项合作工作的目的是利用流式细胞术构建玉米染色体特异性基因文库。 结果应有助于我们的知识，染色体分离技术，这将是广泛有用的，在未来的研究携带有利的易位植物株。 该植物生物学项目通过使美国和东欧的领先专家联合收割机互补人才和在共同感兴趣和能力强的领域汇集研究资源，实现了推进科学知识的计划目标。 ***

9423154 Galbraith This U.S.-Czech research project between Dr. David Galbraith of the University of Arizona and Dr. Jaroslav Dolezel of the Czech Institute of Experimental Botany will examine procedures for cell cycle synchronization in meristem root tip cells, isolation of condensed chromosomes from synchronized cells arrested in metaphase, and the flow sorting of these chromosomes. The objective of this collaborative work is to construct chromosome- specific gene libraries in Zea maize, using flow cytometry. Results should contribute to our knowledge of chromosome separation techniques which will be widely useful in future research on plant strains carrying advantageous translocations. This project in plant biology fulfills the program objective of advancing scientific knowledge by enabling leading experts in the United States and Eastern Europe to combine complementary talents and pool research resources in areas of strong mutual interest and competence. ***