Engineering Research Equipment: Total Internal Reflectance Fluorescence (TIRF) for Bioengineering at Interfaces

9500468 Stebe设备补助金提供资金购买设备，以建造光子计数全内反射荧光（TIRF）仪器和具有视频数字化和光漂白荧光恢复能力的TIRF显微镜，以建立界面生物工程实验室（LBI）。 TIRF方法是非侵入性的、非破坏性的，并且非常适合于动力学研究，因为可以在从秒到小时的时间尺度上收集真实的时间数据。 荧光方法将用于研究三个研究领域的界面动态事件：1）蛋白质和嵌段共聚物与工程双层的相互作用，2）细胞粘附和聚集到仿生表面，3）蛋白质在合成膜中的水凝胶-聚合物界面吸附。 如所描述的那样进行的工作预计将产生界面处的分子和细胞动力学领域的基础知识，并导致新的发现。 ***

9500468 Stebe The equipment grant provides funds to purchase equipment to construct a photon-counting total internal reflectance fluorescence (TIRF) apparatus and a TIRF microscope with video- digitization and photobleaching fluorescence recovery capabilities to establish the Laboratory for Bioengineering at Interfaces (LBI). TIRF methods are non-invasive, non- destructive, and well suited for kinetic studies, as real time data can be gathered on time scales ranging from seconds to hours. The fluorescence methods will be used to study dynamic events at interfaces in three research area: 1) interactions of proteins and block copolymers with engineered bilayers, 2) cellular adhesion and aggregation to biomimetic surfaces, and 3) protein adsorption at hydrogel-polymer interfaces in synthetic membranes. The work carried out as described is expected to produce fundamental knowledge in the field of molecular and cell dynamics at interfaces, and lead to new discoveries. ***