A Genetic Dissection of Ciliate Conjugation

Cole 9507285这项研究的目标是启动对四膜虫结合过程中发育的细胞和遗传分析。为此，我们将，1.)确定接合过程中的发育依赖关系。通过分离突变体或/和物理操作交配条件的组合，2.)通过搜索减轻发育依赖的突变来搜索发育检查点，以及3.)识别和表征参与特定婚姻事件的基因产物的功能。本研究的目的是了解嗜热四膜虫(一种纤毛原生动物)控制其通过与交配有关的复杂细胞质和核事件的细胞和分子机制。与这一过程相关的突变体将被分离并鉴定。此外，交配条件也将受到物理操纵。***

Cole 9507285 The goal of this research is to initiate a cellular and genetic analysis of development during Tetrahymena conjugation. To that end, we will, 1.) Determine developmental dependencies during conjugation. by a combination of isolating mutants or/and physically manipulating the mating conditions, 2.) Search for developmental checkpoints by searching for mutations that relieve the developmental dependency, and 3.) Identify and characterize the functions of gene products that participate in specific conjugal events. %%% The objective of this research is to understand the cellular and molecular mechanisms by which Tetrahymena thermophila, a species of ciliated protozoan, controls its passage through the complex cytoplasmic and nuclear events associated with mating. Mutants relevant to this process will be isolated and characterized. Also the mating conditions will be physically manipulated. ***