Acquisition of Equipment for Automated DNA Sequencing

在国家科学基金会的部分支持下，犹他大学将购买ABI 3377自动DNA测序仪，Perkin-Elmer 9600 PCR仪和Sun sparcstation 20。这台设备将由四位进化遗传学家共享。该设备的获得将大大提高DNA测序和核基因分型的速度和保真度，使科学家们保持在人类进化遗传研究的前沿。最初，该设备将用于至少13个主要研究项目。这些研究包括对南印度种姓人群、密克罗尼西亚人群、阿尔泰西伯利亚人群、美洲原住民人群以及非洲和非裔美国人人群的进化遗传学研究。为了提供一个长期的时间视角，将分析来自美国西南部、大盆地地区、中国北部、北极圈周围和奥匈帝国的古代DNA样本。方正效应在血管性血友病演变中的作用将被研究。线粒体DNA的突变率将通过将大规模测序结果与“DNA芯片”技术进行比较来测量。将分析世界范围内的遗传变异，以检验有关现代智人起源的假设。除了帮助研究之外，该设备还将帮助学生和博士后在他们的进化遗传学训练过程中学习最现代的分子方法。每个研究人员的实验室都有一些学生和研究员。

With partial National Science Foundation support, the University of Utah will purchase an ABI 3377 automated DNA sequencer, an Perkin-Elmer 9600 PCR machine and a Sun SPARCstatioon 20. This equipment will be shared by four evolutionary geneticists. Acquisition of this equipment will greatly increase the speed and fidelity of DNA sequencing and nuclear genotyping and enable the scientists to remain at the cutting edge of human evolutionary genetic research. Initially, the equipment will be used in at least 13 major research projects. These include research on the evolutionary genetics of south Indian caste populations, Micronesian populations, the Altai Siberian population, Native American populations and African and African-American populations. To provide a long-term temporal perspective, ancient DNA samples from the U.S. Southwest, the Great Basin region, North China, the circum-Arctic, and Austro-Hungary will be analyzed. The role of founder effect in the evolution of von Willebrand disease will be investigated. Mutation rates in mitochondrial DNA will be measured by comparing large-scale sequencing results with `DNA chip` technology. Worldwide genetic variation will be analyzed to test hypotheses regarding the origins of modern Homo sapiens. In addition to aiding in research, this equipment will help students and postdoctoral fellows to learn the most modern molecular methods in the course of their training in evolutionary genetics. Each of the researchers' laboratories has a number of students and fellows.