The use of Immunocytochemical Techniques for Phytoplankton Growth Rate Estimation Via Cell Cycle Analysis

9529970 Taylor本研究的主要目标是提出和使用一种易于被科学界采用的方法来测量浮游植物的物种特定生长速率。该方法遵循了从分裂细胞频率技术到diel DNA合成方法，再到测量生长速率的细胞周期方法的发展进程。这种方法使用一种保守的细胞周期蛋白，增殖细胞核抗原（PCNA）作为即将分裂的细胞的标记。荧光抗体用于检测PCNA，标准的荧光显微镜用于检测抗体的存在或不存在，并鉴定浮游植物的种类，从而使该方法可用于广泛的研究人员。这项研究建立并推进了现有的方法，将使海洋生物界的很大一部分受益。浮游生物生物学家的主要目标之一是了解藻华的原因和单个物种作为初级生产者的贡献。该研究将建立一种可靠、快速、经济、可靠的测量物种特定生长速率的方法，并将提供浮游植物细胞周期蛋白的基本信息。* * *

9529970 Taylor The primary goal of this research is to advance and use a method, which can easily by employed by the scientific community, for measuring species specific growth rates of phytoplankton. The method follows a progression of developments from the frequency of dividing cells technique, to the diel DNA synthesis method, to the cell cycle approach for measuring growth rates. With this method a conserved cell cycle protein, PCNA (proliferating cell nuclear antigen) is used as a marker of cells which are about to divide. Fluorescent antibodies are used to detect PCNA, and a standard epifluorescence microscope is used for detecting presence or absence of antibodies and identifying species of phytoplankton, thus making the method available to a broad range of investigators. This research builds upon and advances established methodology and will benefit a large segment of the biological oceanographic community. One of the major goals of plankton biologists is to understand the causes of blooms and the contribution of individual species as primary producers. The research will establish a sound, fast affordable and rugged method for measuring species specific growth rates, and will also provide basic information on cell cycle proteins in phytoplankton. ***