VPW: Development of New Techniques for Chromosome Mapping in Salmonid Fishes

本研究的目的是建立鲑科鱼类染色体定位的新方法，包括使用正弦序列进行染色体鉴定的显带技术、物种特异性和染色体特异性的绘制探针，以及使用粘粒克隆进行基因定位的原位杂交技术。虽然在鲑科鱼类中存在广泛的等位酶基因连锁图谱，但还没有单一拷贝基因被定位到特定的染色体上。染色体图谱的构建将极大地有利于渔业研究，并提高鲑鱼作为发育遗传学、生态遗传学和毒理学研究模式的有用性。准确的图谱还将使我们能够重建染色体进化。这些方法的开发工作将在我1995-1996学年访问华盛顿大学期间进行。华盛顿大学特别适合这项研究，因为渔业学院拥有优秀的鲑鱼研究实验室，以及分子生物学和生物技术系在染色体流动分类和单拷贝原位杂交方面的专业知识。菲利普斯博士正在与渔业学院的教职员工开发染色体图谱和相关项目的合作项目。互动活动包括教授分子系统学研究生研讨会和在分子技术课上做客座演讲。

The objective of this research is to develop new methods for chromosome mapping in salmonid fishes including banding techniques using SINE sequences for chromosome identification, species- specific and chromosome-specific paint probes, and in situ hybridization using cosmid clones for localization of genes. Although there is an extensive linkage map for allozyme loci in salmonids, no single copy genes have been mapped to specific chromosomes. Construction of chromosome maps would greatly benefit fisheries research and enhance the usefulness of salamonid fishes as models for research in developmental genetics, ecological genetics, and toxicology. Accurate maps will also allow us to reconstruct chromosome evolution. Work on development of these methods will be done during my visit to University of Washington in the 1995-1996 academic year. The University of Washington is uniquely suited for this research because the School of Fisheries maintains excellent laboratories for research on salmonid fishes and expertise in the flow sorting of chromosomes and single copy in situ hybridization exists in the department of Molecular Biology and Biotechnology. Dr. Phillips is developing collaborative projects with faculty members in the School of Fisheries on chromosome mapping and related projects. Interactive activities include teaching a graduate seminar in molecular systematics and giving guest lectures in the Molecular Techniques class.