U.S.-Korea Cooperative Research on Lipid Second Messengers in Plant Signal Transduction

9600183 Low该奖项将资助普渡大学化学系Philip S. Low博士与韩国浦项科技大学生命科学系Youngsook Lee博士进行为期24个月的关于植物信号转导中脂质第二信使的合作研究。为了进一步了解脂质衍生的第二信使在植物防御中的作用，研究人员建议(1)测试添加磷脂酶A或C反应产物是否能独立刺激培养细胞和完整植物中的氧化爆发，(2)确定氧化爆发的激发子是否也能激活完整大豆和番茄植物中的磷脂酶A和C。(3)评估在低聚半乳糖醛酸诱导的氧化爆发中有缺陷的突变番茄植株是否在激活这些磷脂酶方面也有缺陷。植物防御系统在暴露于病原体或来自病原体的特定分子（称为激发子）时被激活。迄今为止发现的最快速的植物防御反应之一是氧化爆发，过氧化氢或过氧化氢离子在激发子结合到植物细胞表面受体的几分钟内释放出来的过程。本项目将开展的实验旨在确定磷脂酶A和C是否参与悬浮培养细胞和完整大豆和番茄植株中氧化爆发的信号传导。合作者在这个项目领域的研究经验丰富。然而，Low博士的经验主要是培养细胞试验，而Lee博士则专注于完整的植物系统。他们互补的专业知识将是这项植物防御系统研究的宝贵资产。该项目与美韩合作科学项目的目标相关，该项目旨在通过交流科学信息、思想、技能和技术以及在互惠问题上的合作，提高美韩科学家和工程师之间的合作水平。韩国参与该项目得到了韩国科学工程财团（KOSEF）的支持。该项目在美国国家科学基金（NSF）资助下为PI的研究增加了一个国际合作的维度。mcb - 9303929。＊＊＊

9600183 Low This award provides funds to permit Dr. Philip S. Low, Department of Chemistry, Purdue University, to pursue with Dr. Youngsook Lee, Department of Life Sciences, Pohang University of Science and Technology, Korea, for 24 months, a program of cooperative research on lipid second messengers in plant signal transduction. The collaborators propose to further their understanding of the roles of lipid-derived second messengers in plant defense by (1) testing whether addition of phospholipase A or C reaction products can independently stimulate the oxidative burst in both cultured cells and in intact plants, (2) determining whether elicitors of the oxidative burst also activate phospholipases A and C in intact soybean and tomato plants, and (3) evaluating whether mutant tomato plants that are defective in the oligogalacturonide-induced oxidative burst are also defective in activating these phospholipases. Plant defense systems are activated upon exposure to pathogens or to specific molecules derived from pathogens, termed elicitors. One of the most rapid plant defense responses identified to date is the oxidative burst, a process whereby hydrogen peroxide or peroxide ions are released within minutes of elicitor binding to plant cell surface receptors. The experiments to be undertaken in this project are expected to establish whether phospholipases A and C participate in signaling the oxidative burst in both suspension cultured cells and in intact soybean and tomato plants. The collaborators are experienced in research in the field of this project. Dr. Low's experience, however, is primarily with cultured cell assays, while Dr. Lee has concentrated on intact plant systems. Their complementary expertise will be a valuable asset in this study of plant defense systems. This project is relevant to the objectives of the U.S.-Korea Cooperative Science Program which seeks to increase the level of cooperation between U.S. and Korean scientists and engineer s through the exchange of scientific information, ideas, skills, and techniques and through collaboration on problems of mutual benefit. Korean participation in the project is supported by the Korea Science and Engineering Foundation (KOSEF). This project adds an international cooperative dimension to the PI's research under NSF Grant No. MCB-9303929. ***