U.S.-Cooperative Science: Regulatory Mechanisms of Gibberellin Biosynthesis and response in Arabidopsis

该奖项支持北卡罗来纳州杜克大学的孙太平教授和日本埼玉理化研究所（RIKEN）的Yuji Kamiya博士进行的为期三年的合作研究项目。本项目为拟南芥赤霉素生物合成及响应调控机制的研究。赤霉素（giberellins, GAs）是一种二萜类化合物，其中一些是植物生长调节剂，控制着种子萌发、茎伸长和花发育等多种过程。本项目有三个主要目标：1)研究光对拟南芥GA生物合成途径中第一个基因（GA1）表达的影响，以了解光如何调节响应组织中的GA浓度；2)研究赤霉素生物合成途径中前两种酶（烯醛合成酶A和B）过量产生对植物生长的影响；3)测量新分离的GA反应突变体的GA含量，以确定这些突变是否会改变GA代谢。该项目汇集了构成该过程不同部分的两个实验室的努力：美国人将提供新的GA反应突变体，日本人将进行GA测量。该项目的结果将确定目前尚不清楚的GA生物合成和器官特异性GA反应的调节机制。通过思想和技术的交流，该项目将扩大我们的基础知识基础，促进国际了解与合作。

9603418 Sun This award supports a three year collaborative research project between Professor Tai-ping Sun of Duke University in North Carolina and Dr. Yuji Kamiya of the Institute of Physical and Chemical Research (RIKEN) in Saitama, Japan. The project is a study of the regulatory mechanisms of gibberellin biosynthesis and response in Arabidopsis. Gibberellins (GAs) are diterpenoid compounds, some of which are plant growth regulators, controlling such diverse processes as seed germination, stem elongation, and flower development. There are three major goals of this project: 1) examine the effect of light on the expression of the first gene (GA1) in the Arabidopsis GA biosynthetic pathway to understand how light regulates GA concentrations in responding tissues; 2) examine the effects of overproduction of the first two enzymes (ent- kaurene syntheses A and B) in the GA biosynthetic pathway on plant growth; and 3) measure the GA content of new GA-response mutants that have been isolated to determine whether these mutations alter GA metabolism. The project brings together the efforts of two laboratories that have constituted different parts of the process: the Americans will provide the new GA-response mutants and the Japanese will perform the GA measurements. The results of this project will identify regulatory mechanisms of GA biosynthesis and organ-specific GA responses that are now unclear. Through the exchange of ideas and technology, this project will broaden our base of basic knowledge and promote international understanding and cooperation.