Membrane Lipid Biosynthesis in Chlamydomonas Reinhardtii

[9603626]摩尔某种形式的细胞分裂对所有生物的繁殖至关重要，对更复杂的生命形式的生长和繁殖也至关重要。对这种分化的监管至关重要，而监管的缺失会对所有形式的生命造成可怕的后果。正因为如此，人们对分裂过程进行了广泛的研究，许多方面也越来越了解。这一过程中尚未得到充分研究的一个组成部分是对膜生产的控制。膜包围着细胞外周和细胞器，它们专门负责细胞的特定代谢功能。产生过多、过少或错误的膜成分，或在错误的时间，都会导致子细胞发生畸变。这项研究将致力于测量在细胞分裂周期中膜组成的变化以及伴随这些变化的生物合成/分解代谢过程。将利用光合生物莱茵衣藻，工作将主要集中在其主要的质体外膜脂、磷脂酰乙醇胺和二酰基甘油三甲基高丝氨酸。由于这两种脂质的合成途径尚未确定，因此确定这两种脂质的合成途径和细胞内位置将优先考虑。这个项目是关于膜合成的调节，特别是它们的脂质成分，与细胞分裂和细胞周期有关。如果膜合成和细胞分裂不能很好地协调，可能会导致严重的问题。一种简单的藻类——莱茵衣藻（Chlamydomonas reinhardtii）将被研究，它可以被基因操纵。对两种主要脂类的合成途径进行分析和研究。该项目将提供有关植物如何控制其生长和繁殖的新信息，并可能对作物生产产生长期影响。

9603626 Moore Cell division in some form is central to reproduction of all organisms, and also to growth and reproduction of more complex forms of life. Regulation of this division is crucial, and loss of regulation has dire consequences for all forms of life. Because of this, extensive studies of the division process have been conducted and many aspects are increasingly well understood. One component of the process which has not been sufficiently studied is the control over membrane production. Membranes surround both the periphery and the cellular components, organelles, which specialize in specific metabolic functions of the cell. Production of too much, too little, or the wrong components of the membranes, or at the wrong time, would lead to aberrations in the daughter cells. The research would be devoted to measuring the changes in membrane composition which takes place during a cell division cycle and the biosynthetic/catabolic processes which accompany these changes. The photosynthetic organism, Chlamydomonas reinhardtti, will be utilized and the work will concentrate primarily on its major extraplastid membrane lipids, phosphatidylethanolamine and diacylglyceroltrimethylhomoserine. Since the pathways of synthesis of these two lipids are not defined for C. reinhardtti, determination of the pathways and intracellular locations of synthesis of these two lipids will receive initial priority. This project is concerned with regulation of synthesis of membranes, particularly their lipid components, in relation to cell division and the cell cycle. If membrane synthesis is not properly coordinated with cell division, serious problems could result. A simple alga, Chlamydomonas reinhardtii, that can be manipulated genetically will be studied. The pathway for synthesis of two major lipids will be analyzed and studied. This project will provide new information about how plants control their growth and reproduction and may have a long-term impact on crop production.