Molecular Studies on Antigen Receptor Gene Recombination

Aguera 9603905是一种被认为参与免疫球蛋白同种类型开关重组的淋巴细胞特异性过程的酶，已被鉴定并部分纯化。该酶优先切割开关重组断裂点常见的tgggg和tgagc开关重复基序。从小鼠B淋巴细胞和牛脾细胞的核提取液中，酶活性已纯化到接近均一的水平。对纯化的酶(现在称为Endo-sr)进行了初步的生化表征，支持了该酶是新的这一初步假设。生化特征将继续寻找数据，以验证该酶是假定的开关核酸内切酶活性的假设。为此，将开始根据通过测定纯化酶的多肽组成获得的信息来克隆该基因。进一步研究与淋巴细胞体细胞DNA重排有关的各种因素，包括涉及的酶(S)，不仅有助于更好地理解免疫球蛋白开关重组过程，而且有助于更好地理解哺乳动物细胞体细胞DNA重组的潜在机制。

Aguilera 9603905 An enzyme which is thought to be involved in the lymphocyte-specific process of immunoglobulin isotype-class switch recombination has been identified and partially purified. The enzyme preferentially cleaves tgggg and tgagc switch repeat motifs commonly found at switch recombination breakpoints. The enzyme activity has been purified to near homogeneity from nuclear extracts of murine b lymphocytes and bovine splenocytes. Preliminary biochemical characterization with the purified enzyme, now called endo-sr, support the initial assumption that the enzyme is novel. The biochemical characterization will be continued in the search for data to validate the hypothesis that this enzyme is a putative switch endonuclease activity. To that end, work will be initiated to clone the gene based upon the information gained by determining the peptide composition of the purified enzyme. Further characterization of the various factors, including the involved enzyme(s), implicated in somatic DNA rearrangements in lymphocytes should lead to a better understanding of not only the ig switch recombination process but also the underlying mechanisms of somatic DNA recombination in mammalian cells.