A Preparative Ultracentrifuge and Rotors for Separation and Analysis of Biological Samples

请拨经费购买一台新的制备性超离心机和三个超离心机转子，供蒙大拿州立大学兽医分子生物学系的四个实验室使用。超离心机是生物化学和分子生物学研究中必不可少的设备。目前，本部门只有一台22岁的Beckman L5-65超离心机，无法满足本部门当前和未来的需求。此外，L5正在迅速过时，新的驱动器将不再用于修复这台离心机。去年（奎因博士和帕斯夸尔博士都是1995年入职的）和明年（明年将增加一名教员）增加了对超离心的需求，这进一步强调了我们对所要求设备的需求。该部也缺乏足够的研究人员来进行拟议的研究，这项建议包括3个新的研究人员。目前，该部门有一个较旧的定角转子（10年），一个摆斗转子，一个降额50型转子。这些转子都不能以超过60000转/分的速度运行，可能也不应该使用超过45000转/分的速度。本提案中要求的转子具有更高的速度，可以促进更快、更有效的分离。新设备将主要用于博士的实验室。Quinn, Jutila， Pascual和Burgess，所以这个提案包含了他们使用超离心的项目的描述。这些项目都是由N1H， NSF和USDA等一些机构的研究资助资助的。超离心是这些项目的一部分，用于蛋白质和蛋白质复合物的密度梯度分析、亚细胞分离、膜纯化和DNA纯化。此外，少量的离心机时间（5%）将用于部门课程，以培训学生使用现代生物仪器。

Funds are requested to purchase a new preparative ultracentrifuge and three ultracentrifuge rotors which will be used by four laboratories in the Department of Veterinary Molecular Biology at Montana State University. Ultracentrifuges are essential equipment for all research in biochemistry and molecular biology. Currently, there is only one ultracentrifuge in this Department, a 22 year old Beckman L5-65, which is not capable of meeting the Department's current and future needs. In addition, the L5 is rapidly becoming obsolete, and new drives will no longer be available for repairing this centrifuge. The increased demand for ultracentrifugation resulting from the additional faculty added to this Department over the last year (both Dr. Quinn and Dr. Pascual were hired in 1995) and in the coming year (another faculty member will be added to the Department in the next year) further emphasizes our need for the requested equipment. The Department also lacks sufficient rotors to carry out the proposed research, and this proposal includes 3 new rotors. Currently, this Department has an older fixed-angle rotor (10 years old), a swinging bucket rotor, and a derated Type 50 rotor. None of these rotors are capable of running at speeds over 60,000 r.p.m. and probably should not be used over 45,000 r.p.m. The rotors requested in this proposal are capable of much higher speeds and would facilitate faster and more efficient separations. The new equipment will be used primarily by the labs of Drs. Quinn, Jutila, Pascual, and Burgess, so this proposal contains descriptions of their projects that utilize ultracentrifugation. These projects are all funded by research grants from a number of agencies such as the N1H, NSF, and USDA. Ultracentrifugation is required as part of these projects for density gradient analysis of proteins and protein complexes, subcellular fractionation, membrane purification, and DNA purification. In addition, a small amount of centrifuge time (5%) will be available for use in Departmental classes for training students in the use of modern biological instrumentation.