Two-Stage Fermentation of Whey Permeate for Biodegradable Deicer Production

9613273马修斯生物质废物，如乳制品工业的液体乳清废水，由于其高生化需氧量(BOD)而对环境造成负担。处理这些废物以达到环境标准既是资本密集型，也是能源密集型。由于目前使用的除冰剂对公路基础设施造成广泛的腐蚀相关损害，对供水和土壤造成环境破坏，本提案审查了在生产道路除冰剂替代氯化钠过程中使用乳清渗透液的情况。拟议的研究将通过两阶段细菌发酵过程，研究生物质废物在生产替代除冰剂醋酸钙镁(CMA)和钙镁丙酸盐(CMP)中的使用。此外，还将建立一个数学模型来描述共培养系统中的连续细胞循环发酵。将使用丙酸丙酸杆菌和植物乳杆菌在单一培养和共培养系统中进行生长动力学研究。将确定底物浓度、pH和其他参数对生长和有机酸产量的影响。建立了包括底物抑制动力学和产物抑制动力学的协同生长动力学模型，并从数据中得到了动力学常数。将对共培养和两阶段发酵系统进行连续流动细胞循环发酵研究，以确定产酸量和产品浓度。建立了描述单细胞和共培养系统连续流细胞循环发酵的模型，并用实验数据对模型进行了验证。这项研究将开发将乳清废水转化为有用化学品的创新发酵系统设计所需的数据和基本知识。开发的方法和方法可用于将各种廉价的糖底物和生物质水解物转化为低成本的可生物降解除冰剂的过程中。

9613273 Mathews Biomass wastes such as liquid whey effluents from the dairy industry are a burden on the environment due to their high biochemical oxygen demand (BOD). Treatment of these wastes to meet environmental standards is both capital and energy intensive. This proposal examines the use of whey permeate in the production of a road deicer substitute for sodium chloride, since currently used deicers cause extensive corrosion-related damage to the highway infrastructure and environmental damage to water supplies and soils. The proposed research will examine the use of biomass wastes in the production of substitute deicers, calcium magnesium acetate (CMA) and calcium magnesium propionate (CMP), via a two-stage bacterial fermentation process. In addition, a mathematical model will be to describe continuous cell- recycle fermentation in coculture systems. Growth kinetics studies will be conducted using the bacteria Propionibacterium acidipropionici and Lactobacillus plantarum in monoculture and coculture systems. The effect of substrate concentration, pH and other parameters on growth and organic acid productivity will be determined. A synergistic growth kinetics model that includes substrate and product inhibition kinetics will be developed, and kinetic constants will be obtained from the data. Continuous flow cell-recycle fermentation studies will be conducted for coculture and two-stage fermentation systems to determine acid productivity and product concentrations. A model will be developed to describe continuous flow cell- recycle fermentation for single and coculture systems, and the model will be verified with the experimental data. This research will develop data and basic knowledge required in the design of an innovative fermentation system for the conversion of whey effluents to useful chemicals. The methodology and approach developed can be utilized in processes to convert a variety of inexpensive sugar substrates and biomass hydrolysat es into low-cost biodegradable deicers.