CAREER: A Biomimetic Active Separation Device Based on the Microtubule Motor Protein, Kinesin

摘要该项目是关于将微管马达蛋白（kinesin）纳入纳米制造机器的微分析分离装置的制造。一个原型的有源色谱装置将建立识别，分离和检测特定的DNA片段在一个单一的微机械芯片。溶液中荧光标记的DNA片段将选择性地附着在马达蛋白上，并沿着微通道中的排列微管移动到检测器。该项目在生物分离和化学过程监测方面的潜在影响很大。如果成功，就有可能在制造的机器中模拟细胞内运输。教育计划包括开发一个新的讲座和两个关于蛋白质工程和微加工的实验课程。学生将学习如何将生物学原理应用于常规工程问题。

CTS - 9624907 Russell Stewart University of Utah ABSTRACT The project is on the fabrication of a microanalytical separation device incorporating the microtubular motor protein (kinesin) into nano-fabricated machines. A prototype active chromatography device will be built to recognize, separate and detect specific DNA fragments on a single micromachined chip. A fluorescent-labeled DNA fragment in solution will attach selectively to the motor protein and move along aligned microtubules in a microchannel to a detector. The potential impact of the project is strong in bioseparations and chemical process monitoring. If successful, it may be possible to mimic intracellular transport in fabricated machines. The educational plan includes development of a new lecture and two laboratory courses on protein engineering and microfabrication. Students will be trained to apply biological principles to conventional engineering problems.