Spectroscopic Characterization of Surfactant and Biopolymer Adsorption in Liquid/Liquid Interfaces and Chemically Modified Metal Surfaces

本研究项目由分析和表面化学项目资助，研究了表面活性剂、多肽和寡核苷酸在液/液界面和化学修饰的金属表面吸附形成的单分子膜的设计和光谱表征。在这项为期三年的持续拨款的任期内，科恩教授和他在威斯康星大学的学生将利用分子二次谐波产生（SHG）来探测液/液界面的光谱结构。这些信息有助于理解离子在生物膜上的传输，有助于设计DNA检测和鉴定系统，以及控制由自组装单层形成的仿生系统。在本研究过程中开发的工具和信息在基础分析技术的许多领域具有广泛的应用。在这个研究项目中，研究人员正在开发吸附在液/液界面和金属表面有机覆盖层上的分子取向的光谱探针。利用非线性激光光谱探针、反射吸收红外光谱和等离子体共振光谱研究了这些界面上分子的取向和结构。从这些研究中获得的信息将有助于发展新的分析方法，并为开发信息存储和计算的生物模型奠定基础。

This research project, supported in the Analytical and Surface Chemistry Program, investigates the design and spectroscopic characterization of monolayers formed by the adsorption of surfactants, polypeptides and oligonucleotides at liquid/liquid interfaces and at chemically modified metal surfaces. During the tenure of this three-year continuing grant, Professor Corn and his students at the University of Wisconsin will utilize molecular second harmonic generation (SHG) to probe spectroscopically the structure of the liquid/liquid interface. This information is useful for developing an understanding of ion transport across biological membranes, for the design of systems for DNA detection and identification, and for the control of biomimetic systems formed from self-assembled monolayers. The tools and information developed in the course of this research have wide application in a number of areas of fundamental analytical technology. Spectroscopic probes of the orientation of molecules adsorbed at the liquid/liquid interface and in organic overlayers on metallic surfaces are being developed in this research program. Non- linear laser spectroscopic probes, and reflection absorption infrared and plasmon resonance spectroscopies are used to investigate the orientation and structure of molecules at these interfaces. Information obtained from these studies will be useful in the development of new analytical methodologies, as well as forming a fundamental base for the exploitation of biological models of information storage and computing.