Template Polymerized Chelating Liposomes for Separation and Recovery of Biomolecules from Aqueous Solutions

提案编号：9708007提案类型：研究者发起主要研究者：Roberto F. Guzman, David F. O'Brien所属机构：亚利桑那大学该基金通过化学和运输系统部界面、传输和分离项目的分离和纯化项目子项目授予。主要研究者是亚利桑那大学的Roberto F. Guzman和David F. O'Brien。本研究探索了一种结合分子印迹、固定化金属离子亲和层析以及表面相关模板化金属离子螯合剂修饰的单层脂质体的高表面积优势和独特选择性的蛋白质纯化方法的可行性。该方法旨在为从复杂培养基中纯化蛋白质提供高产率、选择性和维持天然蛋白质构象的手段。该方法是金属离子亲和层析的高选择性使用，具有发展成为具有多个结合位点的生物分子的有效纯化方案的潜力。生物活性大分子的生产，如诊断酶、抗体、激素等，是生物技术的核心。一个主要的问题是这些生物分子的纯化与保留生物活性。这里将要开发的技术有可能解决这些问题。

Abstract Proposal No: 9708007 Proposal Type: Investigator Initiated Principal Investigators: Roberto F. Guzman, David F. O'Brien Affiliation: University of Arizona This grant is awarded through the Separations and Purification Program sub-element of the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigators are Roberto F. Guzman and David F. O'Brien of the University of Arizona. The research explores the feasibility of a method of protein purification that combines the advantages and unique selectivity of molecular imprinting, immobilized metal ion affinity chromatography, and the high surface area of unilamellar liposomes modified with surface associated templated metal ion-chelators. The method aims to provide the means to purify proteins from complex media with high yields, selectivity and maintenance of native protein conformation. The method is a highly selective use of metal ion affinity chromatorgraphy that has the potential to be developed as an efficient purification scheme for biomolecules with multiple binding sites. The production of bioactive macromolecules such as diagnostic enzymes, antibodies, hormones etc., is central to biotechnology. A major issue is the purification of these biomolecules with retention of bioactivity. The technology to be developed here has the potential to address these issues.