Reversible Immobilization of Enzymes and Bioligands Using Metal-Chelate Affinity Interactions

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9309326 Guzman The objective of this research is to study and explore alternative applications of metal chelate interactions, and to develop reversible and specific protein separation methods. Enzymes and inhibitors (bioligands) are to be coupled to soluble bifunctional polymers previously modified with a chelating compound at one of the ends. These modified chelating-polymer-bioligands will then be reversibly attached to a previously fixed metal in a solid or soluble support. This approach will allow the bioligands to be replaced whenever their activity is lost by simply removing the metal ion (and subsequently the chelate-polymer-bioligand) from the system, leaving the initial chelate matrix intact. With this approach, enzymes and specific inhibitors bearing a chelating ligand for the fixed metal can be attached in a reversible manner. ***