Reversible Immobilization of Enzymes and Bioligands Using Metal-Chelate Affinity Interactions

利用金属螯合物亲和相互作用可逆固定酶和生物配体

• 批准号: 9309326
• 负责人: Roberto Guzman
• 金额: $ 10万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-15 至 1997-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9309326&HistoricalAwards=false
• 关键词: Reversible; Immobilization; Enzymes; Bioligands; Using

9309326 Guzman The objective of this research is to study and explore alternative applications of metal chelate interactions, and to develop reversible and specific protein separation methods. Enzymes and inhibitors (bioligands) are to be coupled to soluble bifunctional polymers previously modified with a chelating compound at one of the ends. These modified chelating-polymer-bioligands will then be reversibly attached to a previously fixed metal in a solid or soluble support. This approach will allow the bioligands to be replaced whenever their activity is lost by simply removing the metal ion (and subsequently the chelate-polymer-bioligand) from the system, leaving the initial chelate matrix intact. With this approach, enzymes and specific inhibitors bearing a chelating ligand for the fixed metal can be attached in a reversible manner. ***

9309326 Guzman 这项研究的目的是研究和探索金属螯合物相互作用的替代应用，并开发可逆和特异性的蛋白质分离方法。 酶和抑制剂（生物配体）将与预先在末端之一用螯合化合物修饰的可溶性双功能聚合物偶联。 然后，这些修饰的螯合聚合物生物配体将可逆地附着到固体或可溶性载体中先前固定的金属上。 这种方法将允许生物配体在其活性丧失时通过简单地从系统中去除金属离子（以及随后的螯合物-聚合物-生物配体）来被替换，从而使初始螯合物基质保持完整。 通过这种方法，带有固定金属螯合配体的酶和特异性抑制剂可以以可逆的方式附着。 ***