A Single Molecule Approach to Gene Screening and Expression

这项用于探索性研究的小资助金(SGER)得到了分析和表面化学计划的支持。在这项为期12个月的标准拨款期间，宾夕法尼亚州立大学的尤因教授和戴教授及其学生将把聚DT的单个寡核苷酸分子连接到以阵列形式排列在平板上的小的单分散珠子上。这些阵列将与信使RNA的微量样本杂交，然后反转录将产生一系列cDNA，用于差异筛选和基因表达的定量。使用这种方法，单基因产物可以在单一的自动化过程中通过测序进行筛选和鉴定。许多基因组应用要么涉及少量样本，要么涉及低丰度基因的表达。然后需要通过聚合酶链式反应进行扩增。宾夕法尼亚州立大学尤因教授和戴教授及其学生的工作将允许对一系列cDNA进行单分子检测，从而在单一的自动化过程中对基因表达进行差异筛选和量化。这项工作将对基因组领域的问题产生直接影响。

This Small Grant for Exploratory Research (SGER) is supported in the Analytical and Surface Chemistry Program. During the tenure of this twelve-month standard grant, Professors Ewing and Day and their students at Pennsylvania State University will attach single oligonucleotide molecules of poly dT to small monodispersed beads arranged in arrays on a flat substrate. The arrays will be hybridized with micro samples of messenger RNA and then reverse transcription will produce an array of cDNAs for differential screening and quantification for gene expression. Using this approach single gene products can be screened and identified by sequencing in a single automated process. Many genomic applications involve either small amounts of sample or expression of low abundance genes. Amplification by the polymerase chain reaction is then required. The work of Professors Ewing and Day and their students at Pennsylvania State University will permit single molecule detection on an array of cDNAs permitting differential screening and quantification for gene expression in a single automated process. This work will have immediate impact on problems in genomic fields.