Morphogenesis in Streptomyces

本研究的主要长期目标是了解链霉菌中碳源分解代谢物抑制、抗生素生物合成激活和形态发育之间的调控联系。该项目的一般方法是利用参与复杂碳水化合物利用的分解代谢控制基因作为工具来探索碳利用与形态发生之间的联系。利用这种方法，发现了一类新的突变体，它们在分解代谢控制和形态发生方面存在缺陷，同时也过量产生抗生素。在链霉菌已知的形态突变体中，最多向性的是bldB，在先前的研究中显示，它在碳利用的调节方面存在多向性缺陷。bldB基因产物是S. coelicolor分解代谢抑制、形态发生启动和抗生素生物合成所必需的，研究该基因如何在这些过程的相互联系中发挥作用是当前项目的主要重点。为了促进新突变体的分离、克隆和鉴定，人们正在开发委内瑞拉葡萄球菌的转座子诱变和广义转导方法，以便与颜色葡萄球菌结合使用进行遗传分析。

Westpheling 9722960 The primary long-term objective of this study is to understand the regulatory interconnection between carbon source catabolite repression, activation of antibiotic biosynthesis and morphological development in the Streptomyces bacteria. The general approach of this project has been to use catabolite controlled genes involved in complex carbohydrate utilization as tools to probe the connection between carbon utilization and morphogenesis. Using this approach, a new class of mutants was identified that are at once defective in catabolite control and morphogenesis, and also overproduce antibiotics. The most pleiotropic of the known morphological mutants of Streptomyces, bldB, was shown during the course of previous work to be pleiotropically defective in the regulation of carbon utilization. The bldB gene product is required for catabolite repression, the initiation of morphogenesis and antibiotic biosynthesis in S. coelicolor, and the study of how this gene functions in the interconnection of these processes is a major focus of the current project. To facilitate the isolation, cloning, and characterization of new mutants, transposon mutagenesis and generalized transduction methods in S. venezuelae are being developed to be used in combination with S. coelicolor for genetic analysis.