Development of a Multichannel Time-Resolved Fluorometer for Macro and Mictrostudies

该项目名为“用于宏观和微观研究的多通道时间分辨荧光仪的开发”，旨在更新和提高西奈山时间分辨荧光设备的能力，以提供有关生物大分子及其复合物的结构和动力学的相关信息。该设施目前的仪器部分由1986年NSF奖（DMB-8516318）资助，标题为“用于时间分辨荧光的多用户激光器”。新提案有三个主要目标：更换过时的；有限波长范围脉冲激光系统拥有最先进的脉冲激光系统，能够提供许多研究项目所需的紫外和可见激发波长，构建并实施一个具有三个同时检测通道的新样品室，以提高时间分辨荧光强度和各向异性数据集的收集和精度，将时间分辨荧光与显微镜相结合，以允许研究概述了一个为期三年的计划，其中大部分主要组件的获取/开发将在第一年进行。实施、测试和调试将在接下来的两年内进行。

This project, `Development of a Multichannel Time-Resolved Fluorometer for Macro and Microstudies', is to update and improve the ability of the Time- Resolved Fluorescence Facility at Mount Sinai to provide pertinent information about the structure and dynamics of biological macromolecules and their complexes. The present instrumentation in the Facility was funded in part by a 1986 NSF award (DMB-8516318) entitled `A Multi-User Laser for Time-Resolved Fluorescenc'. The new proposal has three main objectives: replacement of the outdated, limited-wavelength range pulsed laser system with a state-of-the-art pulsed laser system capable of providing the UV and visible excitation wavelengths required by numerous research programs construct and implement a new sample chamber with three simultaneous detection channels to enhance the collection and the precision of time-resolved fluorescence intensity and anisotropy data sets combine time-resolved fluorescence with microscopy to permit studies on 'micro' samples A three year program is outlined, with most of the acquisition/development of the major components to occur in the first year. Implementation, testing, and commissioning will be performed in the following two years.