CAREER: Kinetic and Thermodynamic Studies of Advanced Affinity Techniques for Controlling Interfacial Protein Behavior

这项拨款是通过化学和运输系统部门的界面、运输和分离项目授予的。首席研究员是韦恩州立大学的保罗·范·塔塞尔博士。本研究的目的是阐明蛋白质在亲和表面上的吸附，包括动力学、配体密度和配体间隔长度对蛋白质取向的影响。第二个目标是开发利用电场和特定配体放置来控制蛋白质在表面上放置的技术。PI职业发展的教育方面寻求通过基于网络的互动模块将课程开发与实验室实验相结合。一项拓展计划旨在将本科生与高中生组队，为潜在的科学家和工程师提供更好的教育体验。蛋白质分子在液固界面的固定化是酶催化、生物分离和生物材料涂层的一个重要方面。了解蛋白质如何吸附在表面上并开发新技术来操纵这一吸附过程对生物技术行业具有重要意义。＊＊＊

ABSTRACT CTS-9733310 Paul R. Van Tassel/ Wayne State U. This grant is awarded through the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigator is Dr. Paul Van Tassel of Wayne State University. An objective of the research is the elucidation of protein adsorption onto affinity surfaces, including the effects of kinetics, ligand density and ligand spacer length on protein orientation. A second objective is to develop technology to control protein placement on a surface using electric fields and specific ligand placement. The educational aspects of the PI's career development seek to integrate course development with laboratory experiments through web based interactive modules. An outreach program seeks to team up undergraduates with high school students to provide an enhanced educational experience to potential scientists and engineers. The immobilization of protein molecules at a liquid-solid interface is an important aspect of enzyme catalysis, bioseparations and biomaterials coatings. Understanding how proteins adsorb onto surfaces and developing new techniques to manipulate this adsorption process has significant implications to the biotechnology industry. ***