SGER: Acquisition and Testing of Flow CAM for Plankton Research

这个小型探索性研究(SGER)项目包括购买新开发的流式细胞仪和显微镜(FlowCAM)，并由海洋科学界进行初步测试。FlowCAM有可能极大地加强香农点海洋中心(SPMC)至少三个资助项目的正在进行的研究，从而使该中心成为FlowCAM早期“用户社区”测试的良好场所。FlowCAM被设计用来检查相对稀释液中的浮游藻类和原生动物种群。它使用一种新型透镜，将散射和荧光信息与通过成像路径的细胞的视频分析结合在一起，允许在广泛的焦平面内对单个细胞进行自动成像。它可以快速准确地计算细胞数量，获得大小光谱，并在实际低细胞浓度的放牧实验中检查荧光特性。

9817804StromThis Small Grants for Exploratory Research (SGER) project involves the acquisition of the newly developed Flow Cytometer and Microscope (FlowCAM), and initial testing by the ocean science community. The FlowCAM has the potential to greatly enhance on-going research in at least three funded projects at the Shannon Point Marine Center (SPMC), thus making the Center a good site for the early "user community" testing of the FlowCAM. FlowCAM has been designed to examine planktonic algal and protozoan populations in relatively dilute suspensions. It combines scattering and fluorescence information with video analysis of cells passing the imaging path by using a novel lens which allows automated imaging of single cells in a broad focal plane. It allows quick and accurate counts of cell populations, it obtains size spectra, and examines fluorescent properties during grazing experiments at realistically low cell concentrations.