Contribution of natural microbial communities to the elimination of pathogenic viruses from aquatic environments

致病病毒经常进入自然水生环境。在这里，它们受到一些非生物过程的阻碍和腐烂，例如吸附和辐射。然而，致病病毒也作为外部参与者参与了微生物食物网络。致病病毒在微生物拮抗方面的命运到目前为止还不清楚。拟议的项目旨在调查水环境中单个微生物组在消除致病病毒方面的作用。重点将放在原生动物对病毒的捕食，以及细菌活动对病毒消除的直接和间接影响。这项工作还将首次考虑致病病毒在开放水柱以及沉积物、孔隙水和附着在沉积物基质上的病毒的分布。从使用原生动物、细菌和参考病毒的纯培养物进行良好控制的实验室实验开始，我们随后将在接近自然条件的人工河流系统中进行实地研究，然后在UBA试验场(柏林-马里恩费尔德)的岸边渗透现场进行研究。将在地表水和地下水之间的过渡带确定微生物诱导病毒消除的热点，并与环境因素有关。面对全球变化的情景，所获得的信息可能有助于更好地保护和管理水生态系统、饮用水资源和人类健康。

Pathogenic viruses regularly arrive in natural aquatic environments. Here, they are subject to retardation and decay by a number of abiotic processes such as sorption and radiation. Pathogenic viruses, however, get also involved as extrinsic participators in the microbial food web. The fate of pathogenic viruses with respect to microbial antagonism is not known so far. The proposed project intents to investigate the role of individual groups of microorganisms in aquatic environments in the elimination of pathogenic viruses. Focus will be on protozoan grazing on viruses, as well as direct and indirect effects of bacterial activity to virus elimination. The work will for the first time also consider the distribution of pathogenic viruses in the open water column as well as in sediment porewater and viruses attached to the sediment matrix. Starting with well controlled lab experiments using pure cultures of protozoa, bacteria, and reference viruses, we will subsequently conduct field studies at close to natural conditions in an artificial stream system and later in the bank infiltration site at the UBA test site (Berlin-Marienfelde). Hot spots of microbially induced virus elimination will be identified in the transition zone between surface water and groundwater and related to environmental factors. In face of global change scenarios, the information obtained may help to better protect and manage aquatic ecosystems, drinking water resources and human health.