Acquisition of an Automated DNA Sequencer for Advanced Biomolecular Research

用于高级生物分子研究的自动DNA测序仪的获得DNA序列分析已成为许多生物学研究领域的重要工具。该奖项用于购买Perkin-Elmer ABI 377 DNA测序仪，供夏威夷大学生物学研究人员使用。测序仪将放置在生物技术/分子生物学仪器和培训设施中。该仪器将支持的项目包括：(1)基于聚合酶链式反应的遗传方法鉴定外来有害生物；(2)鉴定费氏弧菌共生诱导的细菌基因；(3)夏威夷植物群的分子系统学；(4)减少转基因表达变异的植物转化技术；(5)定点突变和蛋白质表达系统，以产生新的人血清白蛋白物种。这些项目，以及其他利用新DNA测序仪的项目，不仅将加强夏威夷大学的研究基础设施，还将通过太平洋生物医学研究中心和少数民族机构研究中心计划加强培训工作。

9870959Gabor Mocz University of Hawaii ManoaAcquisition of an Automated DNA Sequencer for Advanced Biomolecular ResearchDNA sequence analysis has become an essential tool in many areas of biological research. This award funds the purchase of a Perkin-Elmer ABI 377 DNA sequencer to be used by researchers in biology at the University of Hawaii. The sequencer will be placed in the Biotechnology/Molecular Biology Instrumentation and Training facility. The projects to be supported by this instrument include (1) exotic pest identification using genetic methods based on the polymerase chain reaction, (2) identification of symbiosis-induced bacterial genes in Vibrio fischeri, (3) molecular systematics of Hawaiian flora, (4) plant transformation technology for reduced variation of transgene expression, and (5) site directed mutagenesis and protein expression system to produce novel human serum albumin species. These projects, and others making use of the new DNA sequencer, will enhance not only the research infrastructure at the University of Hawaii, but also training efforts through the Pacific Biomedical Research Center and the Research Centers in Minority Institutions Program.