Acquisition of Equipment for a Comprehensive Agricultural Genomics Laboratory at Purdue University

为在普渡大学建立一个综合农业基因组学实验室所需的仪器提供资金。在过去的五年中，普渡大学加强了其在植物和微生物遗传学方面的能力。普渡大学遗传学项目由大学资源和美国国家科学基金会植物遗传学培训基金建立和资助。普渡大学现在计划扩大这一项目，建立一个农业基因组学的综合项目。目前用于基因组研究的仪器大量积压，随着农业基因组学计划的扩大，需要增加可用资源以适应预期的需求增长。该奖项将购买的仪器分为三类：(1)DNA序列分析和基因组制图；(2)信息;功能基因组学。资金将提供4台DNA测序仪和相关设备；两台Sparc计算机；两台微阵列机器和额外的显微镜和辅助设备。该设备将支持几种植物和真菌基因组的结构分析。对病原体及其植物宿主中表达的基因进行全面的cDNA克隆，将确定这些相互作用的主要成分。类似的方法将被用于研究次级产物合成、细胞壁发育、细胞骨架相互作用或蛋白质- dna相互作用的分子遗传学。还将开展标准土壤与污染土壤微生物多样性的研究。

Funds are requested for instrumentation needed to establish a Comprehensive Agricultural Genomics Laboratory at Purdue University. Over the past five years, Purdue has strengthened its capabilities in plant and microbial genetics. The Purdue Genetics Program was founded and funded by University resources and an NSF Training Grant in Plant Genetics. Purdue now plans to expand this program with the establishment of a comprehensive program in agricultural genomics. Current instrumentation for genomic research runs with a substantial backlog and there is great need to enhance available resources to accommodate the expected increase in demand as the program in agricultural genomics expands. The instrumentation to be purchased by the award falls into three categories: (1) DNA sequence and analysis and genome mapping; (2) informatics; and (3) functional genomics. Funds will provide four DNA sequencers and associated equipment; two Sparc computers; two micro array machines and additional microscopes and support equipment. This equipment will support analysis of the structure of several plant and fungal genomes. Comprehensive cDNA cloning of genes expressed in both pathogens and their plant hosts will identify major components of these interactions. Similar approach will be taken to study the molecular genetics of secondary product synthesis, cell wall development, cytoskeletal interactions or protein-DNA interactions. Studies will also be initiated on microbial diversity in standard versus polluted soils.