Genomics Analysis Platform for the Bacillus-Invertebrate Cluster CLUSTER: "Experimental Evolution and Natural Variation of Bacillus-Invertebrate Interactions"

寄主-寄生虫的共同进化预计将对两个相互作用的物种的遗传结构产生复杂的后果。这些后果可能涉及与相互作用直接相关的机制(例如，抗药性、致病性)或与可能的共同适应成本相关的机制(例如，生活史权衡)。到目前为止，我们缺乏关于共同进化过程背后的遗传学的确凿信息。基因组分析平台(GAP)旨在对优先方案SPP 1399内芽孢杆菌-无脊椎动物集群的研究项目产生的生物进行全面的基因组和转录分析。我们的方法是基于最先进的测序技术，结合详细的生物信息学和群体遗传数据分析。我们专注于几个实验室控制的进化实验中的生物体，这些实验专门研究宿主-寄生虫相互作用的共同进化适应的动力学。这些生物属于三个完全测序的模式分类群，即微寄生虫苏云金芽孢杆菌及其两个无脊椎动物宿主线虫线虫秀丽线虫和昆虫卡氏拟青霉。因此，它们是理想的无偏见的全基因组筛选方法。基于这种具有已知进化史的独特材料，我们的方法将深入了解宿主-寄生虫共同进化的遗传基础和动力学。

Host-parasite coevolution is predicted to have complex consequences on the genetic architecture of both interacting species. These consequences may relate to the mechanisms that are of direct relevance to the interaction (e.g., resistance, pathogenicity) or associate with a possible cost of coadaptation (e.g. life-history trade-offs). To date, we lack conclusive information on the genetics that underlie the coevolutionary process. The Genomics Analysis Platform (GAP) aims at a comprehensive genomic and transcriptomic analysis of organisms generated by the research projects of the Bacillus-lnvertebrate Cluster within the priority programme SPP 1399. Our approach is based on state-of-the-art sequencing technology combined with detailed bioinformatic and population genetic data analysis. We focus on organisms from several laboratory controlled evolution experiments, which specifically address the dynamics of reciprocal host-parasite coevolutionary adaptations. These organisms belong to three completely sequenced model taxa, the micro-parasite Bacillus thuringiensis and two of its invertebrate hosts, the nematode Caenorhabditis elegans and the insect Tribolium castaneum. They are thus ideally accessible to an unbiased whole genome screening approach. Based on this unique material with a known evolutionary history, our approach will yield in-depth insight into the genetic basis and dynamics of host-parasite coevolution.