SBIR Phase I: Luciferase Directed Substrates for Cell Regulation

这个小企业创新研究第一阶段项目旨在开发能够利用转化植物细胞中萤火虫荧光素酶表达来控制其生长和生理的缀合物的商业用途。研究了一系列植物调节物质d -荧光素偶联物的合成。在第一阶段，我们将测试这些偶联物对表达荧光素酶（luc）活性的特定组织和细胞的吞噬能力。结合物将在组织培养和整株植物中进行测定，以确定是否能以依赖启动子的方式引起局部细胞死亡。这些化合物将广泛用于植物发育和基因表达的控制研究，并有可能通过选择性应用底物和选择luc融合启动子，以可逆的方式产生农业上重要的不育植物物种。

This Small Business Innovation Research Phase I project aims to develop commercial uses for conjugates capable of exploiting firefly luciferase expression in transformed plant cells to control their growth and physiology. The synthesis of a series of D-luciferin conjugates of plant regulatory substances is researched. In Phase I, we will test these conjugates for the ability to ablate specific tissues and cells expressing luciferase (luc) activity. Conjugates will be assayed in tissue culture and in whole plants for the ability to cause localized cell death in a promoter dependent manner. Such compounds will be of general use for plant research on the control of development and gene expression and have the potential to produce agriculturally important sterile plant species in a reversible manner, through the use of selective application of substrate and choice of luc-fusion promoter.