Genetic and Molecular Analysis of Leaf Vein Pattern in Arabidopsis

Sieburth9982876植物维管系统由专门的细胞组成，其功能是运输水分和糖。这些特化的细胞被组织在一起形成静脉，这些静脉相互连接植物的所有部分。维管组织的运输功能对植物的生存至关重要，而这些组织的图案化被认为是对植物生长的限制。尽管维管组织提供了重要的功能，但对控制叶脉模式的遗传和分子机制几乎一无所知。EMS诱导的突变已经鉴定了拟南芥中在叶脉模式中起作用的基因。携带这些突变的纯合子植物表现出各种脉型缺陷，包括血管不连续、脉管丢失、脉管组织紊乱和脉管数量过多。5个叶脉型突变将被表征为表型、遗传和分子特征。表型研究将揭示是否有其他发育缺陷伴随静脉模式缺陷，以及生长素介导的过程是否受到干扰。遗传学研究将包括鉴定双突变体，以评估是否存在叶片发育或激素突变。分子特征将侧重于识别受影响的基因。

Sieburth9982876The plant vascular system is composed of specialized cells that function to transport water and sugars. These specialized cells are organized together into veins, and the veins interconnect all parts of the plant. The transport functions of vascular tissue are vital to plant survival, and the patterning of these tissues is believed to provide limits to plant growth. Despite the critical functions provided by vascular tissue, almost nothing is known about the genetic and molecular mechanisms that control vein pattern.Genes that function in leaf vein pattern in Arabidopsis have already been identified by EMS-induced mutations. Homozygous plants bearing these mutations show a variety of vein patterns defects, including vascular discontinuities, losses of veins, disorganized veins, and excess numbers of veins.Five leaf vein pattern mutants will be characterized phenotypically, genetically, and molecularly. Phenotypic studies will reveal whether other developmental defects accompany the vein pattern defects and whether auxin mediated processes are disrupted. Genetic studies will include characterization of double mutants to assess whether the leaf development or hormone mutants. Molecular characterization will focus on identification of the affected genes.