Strukturbestimmung eines Protein-RNA Komplexes mittels Kernspinresonanz und Kleinwinkelstreuung

人TIP相关蛋白(TAP)是信使核糖核酸(MRNA)的转运因子，转录后介导信使核糖核酸从细胞核释放到细胞质。当被猴D型逆转录病毒感染时，宿主TAP与一个构成运输元件(CTE)形成复合体，通过形成TAP/CTE RNA复合体将病毒mRNA释放到宿主细胞质中。虽然TAP的几个区域的结构已经通过结晶学研究解析，但CTE RNA部分的结构以及它们相互作用的结构基础尚不清楚。在这个项目中，我打算将小角散射(SAS)和核磁共振(核磁共振)相结合，以获得缺乏的结构信息，这对于了解病毒在感染细胞中的增殖具有重大的科学意义。除了科学和医学方面的兴趣，该项目还将使我获得有关核磁共振和SAS技术的方法学和实用知识，当结合使用时，这些技术将非常有希望揭示TAP/CTE RNA和其他多聚体复合体的结构基础。

Human Tip associated protein (TAP) is a transport factor for messenger ribonucleic acids (mRNA), mediating their release from the cell nucleus into the cytoplasm after transcription. Upon infection by simian type D retroviruses, host TAP is complexed with a constitutive transport element (CTE) to allow release of viral mRNA into the host cytoplasm via formation of a TAP/CTE RNA complex. Whereas structures for the several regions of the TAP have been resolved by crystallographic studies, the structure of the CTE RNA part as well as the structural basis of their interaction is unknown. In this project, I intend to combine small angle scattering (SAS) with nuclear magnetic resonance (NMR) in order to obtain lacking structural information, which is of major scientific interest for understanding virus proliferation in infected human cells. Besides its scientific and medical interest, the project will allow me to acquire both methodological and practical know-how concerning the NMR as well as the SAS techniques, which, when used in concert, are very promising to shed light on the structural basis of the TAP/CTE RNA and other multimeric complexes.