ZooGen, a DNA Sequence Database for Calanoid Copepods and Euphausiids: an OBIS Tool for Uniform Standards of Species Identification

建议建立一个国际合作伙伴关系，以建立一个浮游动物基因组数据库(ZooGen)，其中包括甲鱼、桡足类和真甲藻的DNA型序列。ZooGen数据库将被设计成包括这些种群的所有物种，并允许扩展到更多的浮游动物种群。ZooGen的合作伙伴包括来自7个国家的4名私人投资者和13名专家分类顾问。浮游动物样本将从现有的档案收藏中进行分类，与计划中的海洋研究工作协调获得，并在国家海洋水产局进行实地调查期间收集。分类学专家将确认物种的鉴定；DNA测序将在新汉普郡大学进行，在某些情况下，还将在其他合作伙伴的实验室进行。对于每个物种，将确定线粒体细胞色素氧化酶I(MtCOI)基因的一部分的DNA类型序列；根据需要包括多个mtCOI序列，以反映物种内的变异。ZooGen数据库将作为拟议工作的一部分进行设计、创建、管理、维护和分发；这些数据将纳入海洋生物地理信息系统。教育和外联工作将包括：对联合国大学和华盛顿大学的研究生进行培训；2001年夏季在联合国大学举办了分子系统学动手讲习班；私营部门、国际伙伴及其同事和学生之间的交流；除其他外，将ZooGen分子和环境数据纳入OBIS数据库；创建和维护一个项目网站；用ZooGen数据库和简单的检索工具制作光盘；以及在联合国大学海洋补助金教育人员和海洋文件人员的协助下进行非正式教育。

An international partnership is proposed to create a zooplankton genomic (ZooGen) database of DNA type sequences for calanoid copepods and euphausiids. The ZooGen database will be designed to include all species of these groups and to allow expansion to additional zooplankton groups. The ZooGen partnership includes four P.I.s and thirteen expert taxonomic consultants from seven countries. Zooplankton samples will be sorted from existing archival collections, obtained in coordination with planned oceanographic research efforts, and collected during National Marine Fisheries Service field surveys. The taxonomic experts will confirm species' identifications; DNA sequencing will be done at the University of New Hampshire and, in some cases, in other partners' laboratories. For each species, a DNA type sequence will be determined for a portion of the mitochondrial cytochrome oxidase I (mtCOI) gene; multiple mtCOI sequences will be included as necessary to reflect intraspecific variation. The ZooGen database will be designed, created, managed, maintained, and distributed as part of the proposed work; the data will be integrated into the Ocean Biogeographical Information System (OBIS). Education and outreach efforts will include: training of graduate students at UNH and UW; a hands?on Molecular Systematics Workshop at UNH during Summer, 2001; exchanges between the P.Ls, international partners, and their colleagues and students; inclusion of ZooGen molecular and environmental data in the OBIS database, among others; creation and maintenance of a project web site; production of a CD with the ZooGen database and simple search tools; and informal education with the assistance of UNH Sea Grant Educators and Marine Docents.