POWRE: Techniques for Investigating Cytoskeletal Effects on Recombinant Protein Glycosylation

本项目的目的是研究培养条件对重组蛋白生产的影响，特别是细胞黏附对中国仓鼠卵巢细胞(CHO)重组蛋白产量和糖基化模式的影响。POWRE项目的具体目标是：(1)发展研究蛋白质糖基化的技术方面的专门知识，包括高效液相色谱和凝胶电泳；(2)确定哪些探针和成像技术最适合于研究CHO细胞悬浮和贴壁培养的细胞骨架；以及(3)获得初步数据，分析在不同培养条件下产生的组织纤溶酶原激活剂的糖形式，并确定哪些蛋白质糖基化研究技术最适合于未来的研究。

The goal of this project is to investigate the effects of culture conditions on recombinant protein production, specifically the effects of cell adhesion on yield and glycosylation patterns of recombinant proteins produced in Chinese hamster ovary (CHO) cells. The specific goals for the POWRE project are: (1) to develop expertise in the techniques for studying protein glycosylation including high performance liquid chromatography and gel electrophoresis; (2) to determine what probes and imaging techniques are best suited for studies of the cytoskeleton for suspension and adherent cultures of CHO cells; and (3) to obtain preliminary data analyzing the glycoforms of tissue plasminogen activator produced under the different culture conditions and determine which of the techniques for studying protein glycosylation will be best suited for future studies.