Functionality of Dilute-Environment Bacteria

这项工作的目标是了解能够在极稀的溶液中生长的寡杆菌的特性，以便便于对外星生命的检查。虽然在水生系统中生物量占主导地位，但典型的鱼类即使有养殖也很少。新的技术，如单细胞培养，给出了改进的典型形式的例子，概述的战略是产生可以作为详细检查的例子的培养。程序的设计是为了通过观察大量接种了统计上丰富的单细胞的培养物来优化培养能力。出现的种群通过流式细胞仪可视化，物种数量通过使用新的统计公式的分子技术进行分析以评估生存能力。通过培养基修饰物检测对启动生长的敏感性。营养反应是在新的亚努斯动力学的框架内检验的，该动力学将有机体结构与养分封存能力联系起来。关键问题涉及人工污染和底物加速死亡。因此，将使用淡水来消除在介质制备过程中出现的一些与盐有关的问题。还将评估温度和物种-物种之间的相互作用。这些培养物将具有生理和遗传特征。一个目标是获得一种具有代表性的基因测序培养物，以帮助详细了解寡杆菌的功能。

The objective of this effort is to understand properties of oligobacteria that allow growth in extremely dilute solutions in order to facilitate examination for extraterrestrial life. While dominant in terms of biomass in aquatic systems, typical forms are rarely if ever cultivated. New techniques such as growth from single cells give improved examples of typical forms and the strategy outlined is to generate cultures that may taken as examples for detailed examination. Procedures are designed to optimize culturability by observing numerous cultures inoculated with statistically abundant single cells. The populations appearing are visualized by flow cytometry and the number of species are assayed by molecular techniques using novel statistical formulations to assess viability. Sensitivity to the initiation of growth is examined by culture media amendments. Nutrient responses are examined within the framework of novel Janusian kinetics that relate organism construction to nutrient sequestering ability. Key questions involve artifactual contamination and substrate accelerated death. Fresh water will therefore be used to eliminate some salt-associated problems during media preparation. Temperature and species-species interactions will be evaluated as well. The cultures will be characterized physiologically and genetically. One objective is to obtain a representative culture for gene sequencing as an aid to a detailed understanding of the functionality of oligobacteria.