Sensitivity, Distribution, Characterization and Production of Ultramicrobacteria

超微细菌的敏感性、分布、表征和生产

• 批准号: 9019625
• 负责人: Don Button
• 金额: $ 29.2万
• 依托单位: University of Alaska Fairbanks Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9019625&HistoricalAwards=false
• 关键词: Sensitivity; Distribution; Characterization; Production; Ultramicrobacteria

Heterotrophic bacteria recycle carbon, regulate dissolve organics, and comprise much of the biomass of the oceans. Typical organisms are culture resistant, and a factor of 20 smaller with one-tenth the DNA of commonly studied bacteria. To study these oligotrophic ultramicrobacteria, Dr. Button has developed several new techniques. One is high resolution flow cytometry which gives a rapid accurate analysis of populations in seawater and cultures derived from them, including measurements of population, cell size and DNA content per cell. Another is janusian kinetics which bases nutrient transport rates on molecular composition and allows improved descriptions of the ability of whole cells to collect nutrients. Third is a dilution- culture procedure which greatly expands the number of oligotrophic bacterial species that can be cultured. Representatives of what appear to be typical marine bacteria are now on hand. Preliminary data indicate peculiar sensitivity exhibited as a growth-dependency of individual organisms on the presence of others. Dr. Button will to explore these peculiar population dependencies, bacteria and the distributions of organisms that have them, their nutritional requirements, kinetic characteristics, and ability to attain high population densities, in an effort to understand the dynamics and ecology of marine microorganisms in the context of kinetic control of dissolved organics.

异养菌回收碳，调节溶解 有机物，并构成了海洋的大部分生物量。典型 微生物是耐培养的， 通常研究的细菌DNA的十分之一。研究这些 贫营养超微细菌，巴顿博士开发了 几种新技术。一种是高分辨率流式细胞术 它可以快速准确地分析海水中的种群 以及由此衍生的文化，包括测量 群体、细胞大小和每个细胞的DNA含量。另一个是 janusian动力学，它基于营养物质的传输速率， 分子组成，并允许改进的描述， 整个细胞收集营养物质的能力。第三是稀释- 培养程序，大大增加了数量， 可以培养的贫营养细菌物种。 典型海洋细菌的代表是 现在手头上。初步数据显示， 表现为个体生物对环境的生长依赖性， 其他人的存在。巴顿博士将探索这些奇特的 种群依赖性、细菌和 有它们的生物，它们的营养需求，动力学 特性，以及获得高人口密度的能力， 为了了解海洋的动力学和生态学， 微生物在动力学控制的背景下溶解 有机物