REU: Refinement of Instrumentation, Protocols, and Calibrations for the Analysis of Marine Bacteria by Flow Cytometry

阿拉斯加大学海洋科学研究所 将进一步发展流式细胞技术， 生物学和海洋学。 台中科院 流式细胞术是一项技术， 开发用于生物医学研究界， 海洋科学研究。 通过这种技术， 细菌、细胞或其他微观颗粒通过 通过激光束。 不同的细胞和颗粒 可以列举和分类，根据他们如何 发出荧光或响应激光而反应。 分析 可以产生重要的信息， 细胞大小、细胞成分和代谢状况。 这项工作将继续集中在使用流式细胞术 用于研究海洋细菌。 将继续开发适当的流式细胞术 标准和协议。 该实验室还将 供客人调查员使用。 协同 工业将促进进一步的发展和修改 海洋学的基本流式细胞仪 research. 改进和修改将直接 扩大仪器的动态范围， 提高敏感性。 蛋白质测定方法 每个细胞的含量和膜电位也将是 探讨了

The Institute of Marine Science at the University of Alaska will further develop flow cytometric techniques for marine biology and oceanography. The Institute has been instrumental in adapting flow cytometry, a technology developed for use in the biomedical research community, for ocean science research. With this technique, a stream of bacteria, cells, or other microscopic particles is passed through a laser light beam. Different cells and particles can be enumerated and classified according to how they fluoresce or react in response to the laser light. Analysis of the fluoresced signal can yield important information on cell size, cellular constituents, and metabolic condition. This effort will continue to focus on using flow cytometry for studying marine bacteria. Work will continue on developing appropriate flow cytometry standards and protocols. The laboratory will also be available for use by guest investigators. Coordination with industry will promote further development and modifications to basic flow cytometry instrumentation for oceanographic research. Improvements and modifications will be directed towards expanding dynamic range of the instrument and increasing sensitivity. Methods for determining protein content per cell and membrane potential will also be explored.