PECASE: Development of a New Mass Spectrometry-Based Approach for Assaying Protein Stability and Initiation of a High School Science Teacher Outreach Program

摘要提案：0094224日期：2002年3月18日PI：Michael C.菲茨杰拉德研究所：杜克大学这个PECASE奖给杜克大学的Michael Fitzgerald教授是由化学系的分析和表面化学项目支持的。 主要研究目标是开发一种基于质谱的方法来检测蛋白质的稳定性和折叠。 氢-氘交换结合基质辅助激光解吸电离质谱的实验技术与现有的研究蛋白质折叠的技术相比有几个优点。 这项研究的长期目标是开发一种方法来研究多种蛋白质和DNA之间的复杂结合相互作用。 该计划的教育部分是创新和完善的。 菲茨杰拉德博士提出了一个计划，重点是高中科学教师的夏季研讨会。 他与学区科学专家合作制定了该计划，该计划也得到了八位地区校长的支持。这项研究将影响质谱和生物化学领域。 与已建立的方法相比，该方法不受样品纯度的限制，具有更高的灵敏度。 它被设计为自动化和快速。这一研究领域的未来应用可能会影响疾病状态和正常人类健康的研究。

AbstractProposal: 0094224 Date: March 18, 2002PI: Michael C. Fitzgerald Institution: Duke University This PECASE award to Professor Michael Fitzgerald of Duke University is supported by the Analytical and Surface Chemistry Program in the Chemistry Division. The primary research objective is to develop a mass spectrometry based method to examine protein stability and folding. The experimental technique of hydrogen-deuterium exchange combined with matrix assisted laser desorption ionization mass spectrometry has several advantages over existing techniques for studying protein folding. The long-range goals of this research are to develop a method to study complex binding interactions between multiple proteins and DNA. The educational component of this program is both innovative and well developed. Dr. Fitzgerald presents a program focused on a summer workshop for high school science teachers. He collaborated with the school district science specialist in the development of the plan, which has also received the support of eight regional principals. This research will impact the fields of mass spectrometry and biochemistry. Compared to established methods, this method is not limited by sample purity and will have greater sensitivity. It is designed to be automated and fast. Future applications of this line of research could impact the study of both disease state and normal human health.