SBIR Phase I: Thermostable Phage DNA Polymerases: Improved Tools for Genomics Research

0109756 Schoenwehr这个小企业创新研究（SBIR）第一阶段项目将开发新的热稳定噬菌体和古病毒DNA聚合酶，用作DNA扩增、序列分析和单核苷酸多态性（SNP）检测的改进试剂。 这将通过从温泉中直接分离嗜热噬菌体和古病毒基因组、构建表达文库和筛选新型热稳定DNA聚合酶活性来实现。 所提出的方法预计将显着更快速和全面的比传统的方法，已用于酶的发现。 这些传统方法依赖于培养微生物及其同源病毒的限制性中间步骤。相比之下，该项目的拟议方法将允许筛选所有存在的可表达的病毒DNA聚合酶，包括那些来自不可培养的噬菌体的聚合酶，这些噬菌体被认为在环境中占主导地位。该项目的主要商业应用将是向参与基因组学研究的组织销售新型热稳定DNA聚合酶，以用作特定分子生物学方法的改进试剂。

0109756SchoenfeldThis Small Business Innovation Research (SBIR) Phase I project will develop new thermostable bacteriophage and archaeaviral DNA polymerases for use as improved reagents for DNA amplification, sequence analysis and single nucleotide polymorphism (SNP) detection. This will be accomplished by direct isolation of thermophilic bacteriophage and archaeaviral genomes from hot springs, construction of expression libraries and screening for novel thermostable DNA polymerase activities. The proposed approach is expected to be significantly more rapid and comprehensive than traditional approaches that have been used for enzyme discovery. These traditional approaches rely on the limiting intermediate step of culturing a microbe and its cognate virus. In contrast, the proposed approach on this project will allow screening of all expressible viral DNA polymerases present, including those from unculturable phage that is believed to predominate in the environment.The primary commercial application of this project will be in the marketing of novel thermostable DNA polymerases to organizations involved in genomics research for use as improved reagents for specific molecular biology methods..