Arabidopsis 2010: A Sequence-Indexed Library of Insertion Mutations in the Arabidopsis Genome

拟南芥2010：拟南芥基因组中插入突变的序列索引库。 随着整个拟南芥基因组序列的可用性，接下来的挑战之一是揭示这种参考植物中超过25，000个基因的功能。鉴于NSF 2010计划的范围，为了在未来十年内确定所有拟南芥基因的功能，需要一种有效且具有成本效益的方法来确定所有基因中的突变。这个项目的目标是建立一个拟南芥基因组突变的序列索引库。索尔克研究所基因组分析实验室（http：signal.salk.edu）将使用高通量基因组测序方法来鉴定拟南芥基因组中农杆菌T-DNA的插入位点。培养来自Alonso/克罗斯比/Ecker收集的T-DNA转化植物，制备基因组DNA，回收T-DNA侧翼植物DNA并测序。将插入位点序列与拟南芥基因组序列比对，并添加基因注释。数据将通过网络可访问的图形界面-T-DNAExpress-（http：//signal.salk.edu/cgi-bin/tdnaexpress）提供，该界面将提供插入序列数据库的文本和DNA搜索。所有DNA序列将保存在基因库（www.ncbi.nlm.nih.gov）中，并提供给拟南芥信息资源（TAIR）（www.arabidopsis.org）。来自T-DNA插入系的种子将保藏在俄亥俄州州立大学的拟南芥生物资源中心（ABRC）：http://www.biosci.ohiostate.edu/~plantbio/Facilities/abrc/ABRCHOME.HTM。ABRC将向社区传播和分发种子。创建一个可搜索的数据库，其中包含插入位点信息和公共库存中心相应突变株系的可用性，将为研究人员提供随时访问其感兴趣基因中的突变体的机会，从而以前所未有的速度测试有关基因功能的假设。

Arabidopsis 2010: A Sequence-Indexed Library of Insertion Mutations in the Arabidopsis Genome. With the availability of the entire Arabidopsis genome sequence, one of the next challenges is to uncover the functions of the more than 25,000 genes in this reference plant. Given the scope of the NSF 2010 program, to identify the function of all Arabidopsis genes in the next decade, an efficient and cost effective approach is necessary to identify mutations in all genes. The goal of this program is to create a sequence-indexed library of mutations in the Arabidopsis genome. The Salk Institute Genome Analysis Laboratory (http://signal.salk.edu) will use high-throughput genome sequencing methods to identify the sites of insertion of Agrobacterium T-DNA in the Arabidopsis genome. T-DNA transformed plants from the Alonso/Crosby/Ecker collection will be grown, genomic DNA will be prepared, T-DNA flanking plant DNA will be recovered and sequenced. Insertion site sequences will be aligned with the Arabidopsis genome sequence and gene annotation will be added. The data will be made available via a web accessible graphical interface-T-DNAExpress-(http://signal.salk.edu/cgi-bin/tdnaexpress) that will provide both text and DNA searches of the insertion sequence database. All DNA sequences will be deposited into GenBank (www.ncbi.nlm.nih.gov) and also provided to The Arabidopsis Information Resource (TAIR) (www.arabidopsis.org). Seeds from the T-DNA insertion lines will be deposited with the Arabidopsis Biological Resource Center (ABRC) at Ohio State University:http://www.biosci.ohiostate.edu/~plantbio/Facilities/abrc/ABRCHOME.HTM. The ABRC will propagate and distribute seeds to the community. The creation of a searchable database containing the insertion site information and the availability of the corresponding mutant lines in public stock centers will provide researchers with ready access to mutants in their genes of interest, allowing the testing of hypotheses about gene function at an unprecedented rate.