QSB: Analysis of Escherichia coli Transcriptional Regulation

一个贝叶斯统计方法和尺寸排阻DNA微阵列实验，这是相辅相成的，将开发实现操纵子的完整映射。随着操纵子结构的精确确定，调控分析可以准确地解决每个mRNA种类的调控。 该项目解决了在基因组规模上推导调节电路的基本问题。它是将先前的机械知识与大量DNA微阵列数据相结合的早期尝试之一。该项目还试图通过较小的调节蛋白子集来代表全基因组的调节特征。

A Bayesian statistical approach and size-exclusion DNA microarray experimentation, which complement each other, will be developed to achieve the complete mapping of operons. With the precise operon structures determined, the regulational analysis can accurately address the regulation on each mRNA species. This project addresses the fundamental issue of deducing regulatory circuitry at the genomic scale. It is one of the early attempts for combining prior mechanistic knowledge with large amounts of DNA microarray data. The project also attempts to represent the genome-wide regulation features by a smaller subset of regulatory proteins.