Remorin-mediated signal transduction

植物微生物的相互作用需要几种受体、胞浆蛋白和核蛋白协同作用，以触发分子和形态上的反应，以接近共生或病原细菌和真菌。我们最近发现了一种属于紫花苜蓿remorin家族的新型支架蛋白(MtSYMREM1)，它能够与至少三种受体样激酶相互作用，这三种受体是根瘤共生所必需的。虽然我们描述了该蛋白在相互作用的后期阶段的作用，但它在宿主信号感知根瘤因子和早期感染事件中的功能仍不清楚。该项目的第一部分旨在分析SYMREM1蛋白质动力学，重点放在其同源背景中的定位和与其他蛋白质的相互作用上。在第二部分，我们将把我们目前的知识扩展到非豆科植物。利用拟南芥作为模式植物，我们将描述其他亚家族的remorin的特征，并采取全球方法来识别需要remorin蛋白存在的信号通路。详细的生化、遗传和高级显微镜分析将为阐明这些蛋白质的功能提供有价值的信息。

Plant microbe interactions require several receptors, cytosolic and nuclear proteins to act in concert to trigger molecular and morphological responses to approaching symbiotic or pathogenic bacteria and fungi. We recently identified a novel type of scaffold protein belonging to the remorin family in Medicago truncatula (MtSYMREM1) that is able to interact with at least three receptor-like kinases that are essential for root-nodule symbiosis. While we characterised roles of this protein during later stages of the interaction, its functions during host signal perception of rhizobial Nod Factors and early events of infection remains unknown. The first part of the project aims for the analysis of SYMREM1 protein dynamics with a focus on localisation and interactions with other proteins in its homologous background. In the second part, we will extend our current knowledge to non-legumes. Using Arabidopsis thaliana as a model plant we will characterise remorins from other subfamilies and take a global approach to identify signalling pathways that require presence of remorin proteins. Detailed biochemical, genetic and advanced microscopical analyses will provide valuable information to elucidate the functions of these proteins.